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The Next Generation in Membrane Protein Structure Determination

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Attention for Chapter 9: Applications of the BLEND Software to Crystallographic Data from Membrane Proteins.
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Chapter title
Applications of the BLEND Software to Crystallographic Data from Membrane Proteins.
Chapter number 9
Book title
The Next Generation in Membrane Protein Structure Determination
Published in
Advances in experimental medicine and biology, August 2016
DOI 10.1007/978-3-319-35072-1_9
Pubmed ID
Book ISBNs
978-3-31-935070-7, 978-3-31-935072-1
Authors

Pierre Aller, Tian Geng, Gwyndaf Evans, James Foadi

Editors

Isabel Moraes

Abstract

X-ray diffraction from crystals of membrane proteins very often yields incomplete datasets due to, among other things, severe radiation damage. Multiple crystals are thus required to form complete datasets, provided the crystals themselves are isomorphous. Selection and combination of data from multiple crystals is a difficult and tedious task that can be facilitated by purpose-built software. BLEND, in the CCP4 suite of programs for macromolecular crystallography (MX), has been created exactly for this reason. In this chapter the program is described and its workings illustrated by means of data from two membrane proteins.

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The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 50%
Student > Ph. D. Student 1 10%
Student > Bachelor 1 10%
Other 1 10%
Unknown 2 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 30%
Agricultural and Biological Sciences 2 20%
Chemistry 1 10%
Materials Science 1 10%
Engineering 1 10%
Other 0 0%
Unknown 2 20%