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Metabolism in Cancer

Overview of attention for book
Attention for Chapter 2: Lactate-An Integrative Mirror of Cancer Metabolism.
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Chapter title
Lactate-An Integrative Mirror of Cancer Metabolism.
Chapter number 2
Book title
Metabolism in Cancer
Published in
Recent results in cancer research Fortschritte der Krebsforschung Progrès dans les recherches sur le cancer, August 2016
DOI 10.1007/978-3-319-42118-6_2
Pubmed ID
Book ISBNs
978-3-31-942116-2, 978-3-31-942118-6
Authors

Stefan Walenta, Nadine F. Voelxen, Wolfgang Mueller-Klieser, Walenta, Stefan, Voelxen, Nadine F., Mueller-Klieser, Wolfgang

Editors

Thorsten Cramer, Clemens A. Schmitt

Abstract

The technique of induced metabolic bioluminescence imaging (imBI) has been developed to obtain a "snapshot" of the momentary metabolic status of biological tissues. Using cryosections of snap-frozen tissue specimens, imBI combines highly specific and sensitive in situ detection of metabolites with a spatial resolution on a microscopic level and with metabolic imaging in relation to tissue histology. Here, we present the application of imBI in human colorectal cancer. Comparing the metabolic information of one biopsy with that of 2 or 3 biopsies per individual cancer, the classification into high versus low lactate tumors, reflecting different glycolytic activities, based on a single biopsy was in agreement with the result from multiple biopsies in 83 % of all cases. We further demonstrate that the metabolic status of tumor tissue can be preserved at least over 10 years by storage in liquid nitrogen, but not by storage at -80 °C. This means that tissue banking with long-term preservation of the metabolic status is possible at -180 °C, which may be relevant for studies on long-term survival of cancer patients. As with other tumor entities, tissue lactate concentration was shown to be correlated with tumor development and progression in colorectal cancer. At first-time diagnosis, lactate values were low in rectal normal tissue and adenomas, were significantly elevated to intermediate levels in non-metastatic adenocarcinomas, and were very high in carcinomas with distant metastasis. There was an inverse behavior of tissue glucose concentration under corresponding conditions. The expression level of monocarboxylate transporter-4 (MCT4) was positively correlated with the tumor lactate concentration and may thus contribute to high lactate tumors being associated with a high degree of malignancy.

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 4 19%
Student > Ph. D. Student 3 14%
Student > Master 3 14%
Student > Bachelor 1 5%
Lecturer 1 5%
Other 1 5%
Unknown 8 38%
Readers by discipline Count As %
Medicine and Dentistry 5 24%
Agricultural and Biological Sciences 1 5%
Immunology and Microbiology 1 5%
Physics and Astronomy 1 5%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Other 4 19%
Unknown 8 38%