Patient-Specific Induced Pluripotent Stem Cell Models
Humana Press
Chapter title |
Patient-Specific Induced Pluripotent Stem Cell Models
|
---|---|
Chapter number | 225 |
Book title |
Patient-Specific Induced Pluripotent Stem Cell Models
|
Published in |
Methods in molecular biology, July 2015
|
DOI | 10.1007/7651_2015_225 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3033-3, 978-1-4939-3034-0
|
Authors |
Li, Yao, Nguyen, Huy V, Tsang, Stephen H, Yao Li, Huy V. Nguyen, Stephen H. Tsang |
Editors |
Andras Nagy, Kursad Turksen |
Abstract |
The generation of patient-specific induced pluripotent stem (iPS) cells permits the development of next-generation patient-specific systems biology models reflecting personalized genomics profiles to better understand pathophysiology. In this chapter, we describe how to create a patient-specific iPS cell line. There are three major steps: (1) performing a skin biopsy procedure on the patient; (2) extracting human fibroblast cells from the skin biopsy tissue; and (3) reprogramming patient-specific fibroblast cells into the pluripotent stem cell stage. |
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