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Mendeley readers
Chapter title |
Transgene-Free Disease-Specific iPSC Generation from Fibroblasts and Peripheral Blood Mononuclear Cells.
|
---|---|
Chapter number | 278 |
Book title |
Patient-Specific Induced Pluripotent Stem Cell Models
|
Published in |
Methods in molecular biology, July 2015
|
DOI | 10.1007/7651_2015_278 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3033-3, 978-1-4939-3034-0
|
Authors |
Kerem Fidan, Ayyub Ebrahimi, Özlem H. Çağlayan, Burcu Özçimen, Tamer T. Önder |
Editors |
Andras Nagy, Kursad Turksen |
Abstract |
Induced pluripotent stem cells (iPSCs) offer great promise as tools for basic biomedical research, disease modeling, and drug screening. In this chapter, we describe the generation of patient-specific, transgene-free iPSCs from skin biopsies and peripheral blood mononuclear cells through electroporation of episomal vectors and growth under two different culture conditions. The resulting iPSC lines are characterized with respect to pluripotency marker expression through immunostaining, tested for transgene integration by PCR, and assayed for differentiation capacity via teratoma formation. |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 22% |
Student > Master | 4 | 17% |
Student > Bachelor | 3 | 13% |
Researcher | 3 | 13% |
Student > Doctoral Student | 2 | 9% |
Other | 1 | 4% |
Unknown | 5 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 39% |
Agricultural and Biological Sciences | 4 | 17% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 9% |
Medicine and Dentistry | 2 | 9% |
Chemical Engineering | 1 | 4% |
Other | 0 | 0% |
Unknown | 5 | 22% |