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Clinical Proteomics

Overview of attention for book
Cover of 'Clinical Proteomics'

Table of Contents

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    Book Overview
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    Chapter 1 Biological Sample Collection for Clinical Proteomics: Existing SOPs.
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    Chapter 2 Targeting the proteome of cellular fractions: focus on secreted proteins.
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    Chapter 3 Preparation of urinary exosomes: methodological issues for clinical proteomics.
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    Chapter 4 Sample treatment methods involving combinatorial Peptide ligand libraries for improved proteomes analyses.
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    Chapter 5 Glycoprotein Enrichment Method Using a Selective Magnetic Nano-Probe Platform (MNP) Functionalized with Lectins.
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    Chapter 6 The Latest Advancements in Proteomic Two-dimensional Gel Electrophoresis Analysis Applied to Biological Samples.
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    Chapter 7 2DE Maps in the Discovery of Human Autoimmune Kidney Diseases: The Case of Membranous Glomerulonephritis.
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    Chapter 8 MALDI-Imaging Mass Spectrometry on Tissues.
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    Chapter 9 Laser Capture Microdissection of Fluorescently Labeled Amyloid Plaques from Alzheimer’s Disease Brain Tissue for Mass Spectrometric Analysis
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    Chapter 10 Urine Sample Preparation and Fractionation for Global Proteome Profiling by LC-MS.
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    Chapter 11 Methods in capillary electrophoresis coupled to mass spectrometry for the identification of clinical proteomic/peptidomic biomarkers in biofluids.
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    Chapter 12 Quantification of proteins in urine samples using targeted mass spectrometry methods.
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    Chapter 13 Statistical issues in the design and planning of proteomic profiling experiments.
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    Chapter 14 Integrating Proteomics Profiling Data Sets: A Network Perspective
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    Chapter 15 The European medicines agency experience with biomarker qualification.
Attention for Chapter 6: The Latest Advancements in Proteomic Two-dimensional Gel Electrophoresis Analysis Applied to Biological Samples.
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Chapter title
The Latest Advancements in Proteomic Two-dimensional Gel Electrophoresis Analysis Applied to Biological Samples.
Chapter number 6
Book title
Clinical Proteomics
Published in
Methods in molecular biology, October 2014
DOI 10.1007/978-1-4939-1872-0_6
Pubmed ID
25384742
Book ISBNs
978-1-4939-1871-3, 978-1-4939-1872-0
Authors

Laura Santucci, Maurizio Bruschi, Gian Marco Ghiggeri, Giovanni Candiano, Santucci, Laura, Bruschi, Maurizio, Ghiggeri, Gian Marco, Candiano, Giovanni

Editors

Antonia Vlahou, Manousos Makridakis

Abstract

Two-dimensional gel electrophoresis (2DE) is one of the fundamental approaches in proteomics for the separation and visualization of complex protein mixtures. Proteins can be analyzed by 2DE using isoelectric focusing (IEF) in the first dimension, combined to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the second dimension, gel staining (silver and Coomassie), image analysis, and 2DE gel database. High-resolution 2DE can resolve up to 5,000 different proteins simultaneously (∼2,000 proteins routinely), and detect and quantify <1 ng of protein per spot. Here, we describe the latest developments for a more complete analysis of biological fluids.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 11%
Student > Doctoral Student 1 11%
Student > Master 1 11%
Unknown 6 67%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 11%
Sports and Recreations 1 11%
Unknown 7 78%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 July 2015.
All research outputs
#17,731,702
of 22,770,070 outputs
Outputs from Methods in molecular biology
#7,189
of 13,090 outputs
Outputs of similar age
#174,124
of 258,575 outputs
Outputs of similar age from Methods in molecular biology
#47
of 126 outputs
Altmetric has tracked 22,770,070 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 258,575 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 126 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.

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