Chapter title |
Molecular Genetics of Asthma
|
---|---|
Chapter number | 8 |
Book title |
Molecular Genetics of Asthma
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3652-6_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3650-2, 978-1-4939-3652-6
|
Authors |
García-Solaesa, Virginia, Abad, Sara Ciria, Virginia García-Solaesa, Sara Ciria Abad |
Abstract |
Western blotting is used to analyze proteins after being separated by electrophoresis and subsequently electro-transferred to a membrane. Once immobilized, a specific protein can be identified through its reaction with a labeled antibody or antigen. It is a methodology commonly used in biomedical research such as asthma studies, to assess the pathways of inflammatory mediators involved in the disease.Here, we describe an example of western blotting to determine the factors involved in asthma. In this chapter, the methodology of western blotting is reviewed, paying attention on potential problems and giving interesting recommendations. |
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