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Mendeley readers
| Chapter title |
Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line
|
|---|---|
| Chapter number | 14 |
| Book title |
Molecular Genetics of Asthma
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3652-6_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3650-2, 978-1-4939-3652-6
|
| Authors |
Elena Marcos-Vadillo, Asunción García-Sánchez, Marcos-Vadillo, Elena, García-Sánchez, Asunción |
| Abstract |
The development of reporters systems has simplified the study of promoter activity in different areas of knowledge, and represents an easy and fast approach to study genetic variations. In this chapter, we show a transfection protocol of A549 lung epithelial cells with a reporter vector, using the Luciferase-Renilla dual system for studying the variations caused by several polymorphisms in the promoter region of a gene. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 33% |
| Student > Ph. D. Student | 1 | 17% |
| Researcher | 1 | 17% |
| Student > Bachelor | 1 | 17% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 2 | 33% |
| Environmental Science | 1 | 17% |
| Agricultural and Biological Sciences | 1 | 17% |
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Unknown | 1 | 17% |