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Mendeley readers
| Chapter title |
Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell
|
|---|---|
| Chapter number | 12 |
| Book title |
Molecular Genetics of Asthma
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3652-6_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3650-2, 978-1-4939-3652-6
|
| Authors |
Elena Marcos-Vadillo, Asunción García-Sánchez, Marcos-Vadillo, Elena, García-Sánchez, Asunción |
| Abstract |
Experimentation with cell cultures is widespread in different areas of basic science as well as in the development of biotechnology applied to medicine. Cellular models are applied to the study of respiratory diseases. In this chapter, we present a protocol of basic cell culture using A549 Human lung adenocarcinoma epithelial cells as model. Corticosteroid therapy is used to treat respiratory diseases such asthma. Thus, we also describe a protocol of lung epithelial cell culture treated with dexamethasone to illustrate an example of monitoring the effects of a drug in a lung cell culture assay. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 30% |
| Student > Master | 2 | 20% |
| Other | 1 | 10% |
| Student > Ph. D. Student | 1 | 10% |
| Researcher | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 20% |
| Biochemistry, Genetics and Molecular Biology | 2 | 20% |
| Medicine and Dentistry | 2 | 20% |
| Immunology and Microbiology | 1 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 2 | 20% |