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Histone Deacetylases

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Cover of 'Histone Deacetylases'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 A Sensitive and Flexible Assay for Determining Histone Deacetylase 1 (HDAC1) Activity
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    Chapter 2 Histone Deacetylases
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    Chapter 3 Histone Deacetylases
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    Chapter 4 Histone Deacetylases
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    Chapter 5 Approaches for Studying the Subcellular Localization, Interactions, and Regulation of Histone Deacetylase 5 (HDAC5)
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    Chapter 6 Analysis of Expression and Functions of Histone Deacetylase 6 (HDAC6)
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    Chapter 7 Histone Deacetylases
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    Chapter 8 Histone Deacetylases
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    Chapter 9 Visualization of HDAC9 Spatiotemporal Subcellular Localization in Primary Neuron Cultures
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    Chapter 10 Expression and Function of Histone Deacetylase 10 (HDAC10) in B Cell Malignancies
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    Chapter 11 Histone Deacetylases
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    Chapter 12 Sirtuin1 (SIRT1) in the Acetylation of Downstream Target Proteins
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    Chapter 13 Protocols for Cloning, Expression, and Functional Analysis of Sirtuin2 (SIRT2)
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    Chapter 14 Cloning and Characterization of Sirtuin3 (SIRT3)
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    Chapter 15 Identification of Sirtuin4 (SIRT4) Protein Interactions: Uncovering Candidate Acyl-Modified Mitochondrial Substrates and Enzymatic Regulators
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    Chapter 16 Generation and Purification of Catalytically Active Recombinant Sirtuin5 (SIRT5) Protein
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    Chapter 17 Sirtuin 6 (SIRT6) Activity Assays
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    Chapter 18 Histone Deacetylases
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    Chapter 19 Histone Deacetylases
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    Chapter 20 Assessment of the Antiproliferative Activity of a BET Bromodomain Inhibitor as Single Agent and in Combination in Non-Hodgkin Lymphoma Cell Lines
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    Chapter 21 Histone Deacetylases
  23. Altmetric Badge
    Chapter 22 Erratum to: Functional Analysis of Histone Deacetylase 11 (HDAC11)
Attention for Chapter 2: Histone Deacetylases
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Chapter title
Histone Deacetylases
Chapter number 2
Book title
Histone Deacetylases
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3667-0_2
Pubmed ID
Book ISBNs
978-1-4939-3665-6, 978-1-4939-3667-0
Authors

Citro, Simona, Chiocca, Susanna, Simona Citro, Susanna Chiocca

Abstract

Small ubiquitin-related modifier (SUMO) is an ubiquitin-like protein that is covalently attached to a variety of target proteins and has a significant role in their regulation. HDAC2 is an important epigenetic regulator, promoting the deacetylation of histones and non-histone proteins. HDAC2 has been shown to be modified by SUMO1 at lysine 462. Here we describe how to detect SUMO modification of endogenous HDAC2 in mammalian cells by immunoblotting. Although in this chapter we use this method to detect HDAC2 modification in mammalian cells, this protocol can be used for any cell type or for any protein of interest.

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Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 33%
Student > Bachelor 1 33%
Lecturer > Senior Lecturer 1 33%
Readers by discipline Count As %
Unspecified 1 33%
Biochemistry, Genetics and Molecular Biology 1 33%
Agricultural and Biological Sciences 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 June 2016.
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#20,330,976
of 22,875,477 outputs
Outputs from Methods in molecular biology
#9,917
of 13,131 outputs
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#330,739
of 393,698 outputs
Outputs of similar age from Methods in molecular biology
#1,054
of 1,471 outputs
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