Histone Deacetylases
Springer New York
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Timeline
Mendeley readers
| Chapter title |
Sirtuin 6 (SIRT6) Activity Assays
|
|---|---|
| Chapter number | 17 |
| Book title |
Histone Deacetylases
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3667-0_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3665-6, 978-1-4939-3667-0
|
| Authors |
Minna Rahnasto-Rilla, Maija Lahtela-Kakkonen, Ruin Moaddel, Rahnasto-Rilla, Minna, Lahtela-Kakkonen, Maija, Moaddel, Ruin |
| Abstract |
SIRT6 has been shown to possess weak deacetylation, mono-ADP-ribosyltransferase activity, and deacylation activity in vitro. SIRT6 selectively deacetylates H3K9Ac and H3K56Ac. Several SIRT6 assays have been developed including HPLC assays, fluorogenic assays, FRET, magnetic beads, in silico, and bioaffinity chromatography assays. Herein, we describe detailed protocols for the HPLC based activity/inhibition assays, magnetic beads deacetylation assays, bioaffinity chromatographic assays as well as fluorogenic and in silico assays. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 36% |
| Researcher | 4 | 29% |
| Student > Bachelor | 1 | 7% |
| Professor > Associate Professor | 1 | 7% |
| Unknown | 3 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 36% |
| Agricultural and Biological Sciences | 3 | 21% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 14% |
| Medicine and Dentistry | 1 | 7% |
| Unknown | 3 | 21% |