Chapter title |
Assessment of the Antiproliferative Activity of a BET Bromodomain Inhibitor as Single Agent and in Combination in Non-Hodgkin Lymphoma Cell Lines
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Chapter number | 20 |
Book title |
Histone Deacetylases
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3667-0_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3665-6, 978-1-4939-3667-0
|
Authors |
Elena Bernasconi, Eugenio Gaudio, Ivo Kwee, Francesco Bertoni, Bernasconi, Elena, Gaudio, Eugenio, Kwee, Ivo, Bertoni, Francesco |
Abstract |
To evaluate the antiproliferative activity of a novel BET Bromodomain inhibitor as single agent and in combination with the BTK inhibitor ibrutinib in non-Hodgkin lymphoma cell lines, we performed the MTT proliferation assay. This assay is based on the direct correlation between absorbance (measured colorimetrically at a wavelength of 570 nm) and cell proliferation. Thiazolyl Blue Tetrazolium Blue (MTT) is a yellowish solution that distinguishes between proliferating and dead cells since it is converted to water-insoluble MTT-formazan of dark blue color by mitochondrial dehydrogenases of living cells only. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 1 | 20% |
Other | 1 | 20% |
Student > Master | 1 | 20% |
Unknown | 2 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 20% |
Materials Science | 1 | 20% |
Chemistry | 1 | 20% |
Unknown | 2 | 40% |