| Chapter title |
Histone Deacetylases
|
|---|---|
| Chapter number | 3 |
| Book title |
Histone Deacetylases
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3667-0_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3665-6, 978-1-4939-3667-0
|
| Authors |
Wang, Jian-Qiu, Wu, Min-Zu, Wu, Kou-Juey, Jian-Qiu Wang, Min-Zu Wu, Kou-Juey Wu |
| Abstract |
Epigenetics plays a key role in gene expression control. Histone modifications including acetylation/deacetylation or methylation/demethylation are major epigenetic mechanisms known to regulate epithelial-mesenchymal transition (EMT)-associated gene expression during hypoxia-induced cancer metastasis. Chromatin immunoprecipitation (ChIP) assay is a powerful tool for investigation of histone modification patterns of genes of interest. In this chapter, we describe a protocol that uses chromatin immunoprecipitation (ChIP) to analyze the epigenetic regulation of EMT marker genes by deacetylation of acetylated Histone 3 Lys 4 (H3K4Ac) under hypoxia in a head and neck cancer cell line FaDu cells. Not only a method of ChIP coupled by real-time quantitative PCR but also the detailed conditions are provided based on our previously published studies. |
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Demographic breakdown
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| Members of the public | 2 | 100% |
Mendeley readers
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| Other | 1 | 8% |
| Other | 0 | 0% |
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| Unknown | 5 | 42% |