| Chapter title |
Protein Targeting Compounds
|
|---|---|
| Chapter number | 3 |
| Book title |
Protein Targeting Compounds
|
| Published in |
Advances in experimental medicine and biology, January 2016
|
| DOI | 10.1007/978-3-319-32805-8_3 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-932804-1, 978-3-31-932805-8
|
| Authors |
Tomszak, Florian, Weber, Susanne, Zantow, Jonas, Schirrmann, Thomas, Hust, Michael, Frenzel, André, Florian Tomszak, Susanne Weber, Jonas Zantow, Thomas Schirrmann, Michael Hust, André Frenzel |
| Abstract |
Since the development of therapeutic antibodies the demand of recombinant human antibodies is steadily increasing. Traditionally, therapeutic antibodies were generated by immunization of rat or mice, the generation of hybridoma clones, cloning of the antibody genes and subsequent humanization and engineering of the lead candidates. In the last few years, techniques were developed that use transgenic animals with a human antibody gene repertoire. Here, modern recombinant DNA technologies can be combined with well established immunization and hybridoma technologies to generate already affinity maturated human antibodies. An alternative are in vitro technologies which enabled the generation of fully human antibodies from antibody gene libraries that even exceed the human antibody repertoire. Specific antibodies can be isolated from these libraries in a very short time and therefore reduce the development time of an antibody drug at a very early stage.In this review, we describe different technologies that are currently used for the in vitro and in vivo generation of human antibodies. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 5 | 19% |
| Student > Ph. D. Student | 5 | 19% |
| Researcher | 5 | 19% |
| Student > Bachelor | 2 | 8% |
| Other | 2 | 8% |
| Other | 1 | 4% |
| Unknown | 6 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 38% |
| Agricultural and Biological Sciences | 6 | 23% |
| Immunology and Microbiology | 1 | 4% |
| Engineering | 1 | 4% |
| Unknown | 8 | 31% |