| Chapter title |
Focus on Bio-Image Informatics
|
|---|---|
| Chapter number | 4 |
| Book title |
Focus on Bio-Image Informatics
|
| Published in |
Advances in anatomy embryology and cell biology, May 2016
|
| DOI | 10.1007/978-3-319-28549-8_4 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-928547-4, 978-3-31-928549-8
|
| Authors |
Paparelli, Laura, Corthout, Nikky, Pavie, Benjamin, Annaert, Wim, Munck, Sebastian, Laura Paparelli, Nikky Corthout, Benjamin Pavie, Wim Annaert, Sebastian Munck |
| Editors |
Winnok H. De Vos, Sebastian Munck, Jean-Pierre Timmermans |
| Abstract |
The spatial distribution of proteins within the cell affects their capability to interact with other molecules and directly influences cellular processes and signaling. At the plasma membrane, multiple factors drive protein compartmentalization into specialized functional domains, leading to the formation of clusters in which intermolecule interactions are facilitated. Therefore, quantifying protein distributions is a necessity for understanding their regulation and function. The recent advent of super-resolution microscopy has opened up the possibility of imaging protein distributions at the nanometer scale. In parallel, new spatial analysis methods have been developed to quantify distribution patterns in super-resolution images. In this chapter, we provide an overview of super-resolution microscopy and summarize the factors influencing protein arrangements on the plasma membrane. Finally, we highlight methods for analyzing clusterization of plasma membrane proteins, including examples of their applications. |
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Demographic breakdown
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Mendeley readers
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Demographic breakdown
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| Researcher | 14 | 23% |
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| Student > Master | 5 | 8% |
| Other | 7 | 11% |
| Unknown | 4 | 6% |
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