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Gene Synthesis

Overview of attention for book
Cover of 'Gene Synthesis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Building block synthesis using the polymerase chain assembly method.
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    Chapter 2 Oligonucleotide assembly in yeast to produce synthetic DNA fragments.
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    Chapter 3 TopDown Real-Time Gene Synthesis
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    Chapter 4 De Novo DNA Synthesis Using Single-Molecule PCR
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    Chapter 5 SLIC: A Method for Sequence- and Ligation-Independent Cloning.
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    Chapter 6 Assembly of Standardized DNA Parts Using BioBrick Ends in E. coli.
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    Chapter 7 Assembling DNA Fragments by USER Fusion
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    Chapter 8 Fusion PCR via Novel Overlap Sequences
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    Chapter 9 Using recombineering to generate point mutations: the oligonucleotide-based "hit and fix" method.
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    Chapter 10 Using Recombineering to Generate Point Mutations:galK-Based Positive-Negative Selection Method.
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    Chapter 11 Assembling Large DNA Segments in Yeast
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    Chapter 12 Recursive construction of perfect DNA molecules and libraries from imperfect oligonucleotides.
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    Chapter 13 Cloning whole bacterial genomes in yeast.
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    Chapter 14 Production of infectious poliovirus from synthetic viral genomes.
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    Chapter 15 In silico design of functional DNA constructs.
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    Chapter 16 Using DNAWorks in Designing Oligonucleotides for PCR-Based Gene Synthesis
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    Chapter 17 De Novo Gene Synthesis Design Using TmPrime Software
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    Chapter 18 Design-A-Gene with GeneDesign.
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    Chapter 19 Leading a Successful iGEM Team.
  21. Altmetric Badge
    Chapter 20 The Build-a-Genome Course.
  22. Altmetric Badge
    Chapter 21 DNA Synthesis Security
Attention for Chapter 6: Assembly of Standardized DNA Parts Using BioBrick Ends in E. coli.
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Chapter title
Assembly of Standardized DNA Parts Using BioBrick Ends in E. coli.
Chapter number 6
Book title
Gene Synthesis
Published in
Methods in molecular biology, February 2012
DOI 10.1007/978-1-61779-564-0_6
Pubmed ID
22328426
Book ISBNs
978-1-61779-563-3, 978-1-61779-564-0
Authors

Ho-Shing O, Lau KH, Vernon W, Eckdahl TT, Campbell AM, Olivia Ho-Shing, Kin H. Lau, William Vernon, Todd T. Eckdahl, A. Malcolm Campbell, Ho-Shing, Olivia, Lau, Kin H., Vernon, William, Eckdahl, Todd T., Campbell, A. Malcolm

Abstract

Synthetic biologists have adopted the engineering principle of standardization of parts and assembly in the construction of a variety of genetic circuits that program living cells to perform useful tasks. In this chapter, we describe the BioBrick standard as a widely used method. We present methods by which new BioBrick parts can be designed and produced, starting with existing clones, naturally occurring DNA, or de novo. We detail the procedures by which BioBrick parts can be assembled into construction intermediates and into biological devices. These protocols are based on our experience in conducting synthetic biology research with undergraduate students in the context of the iGEM competition.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 3%
United States 1 3%
Greece 1 3%
Unknown 29 91%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 22%
Student > Ph. D. Student 5 16%
Student > Master 4 13%
Professor 3 9%
Professor > Associate Professor 3 9%
Other 6 19%
Unknown 4 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 41%
Biochemistry, Genetics and Molecular Biology 7 22%
Chemical Engineering 2 6%
Engineering 2 6%
Nursing and Health Professions 1 3%
Other 2 6%
Unknown 5 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 February 2012.
All research outputs
#19,394,751
of 24,701,594 outputs
Outputs from Methods in molecular biology
#7,973
of 13,877 outputs
Outputs of similar age
#204,326
of 260,373 outputs
Outputs of similar age from Methods in molecular biology
#318
of 475 outputs
Altmetric has tracked 24,701,594 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,877 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 260,373 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 475 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.

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