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X Demographics
Mendeley readers
Attention Score in Context
| Chapter title |
Oligonucleotide assembly in yeast to produce synthetic DNA fragments.
|
|---|---|
| Chapter number | 2 |
| Book title |
Gene Synthesis
|
| Published in |
Methods in molecular biology, February 2012
|
| DOI | 10.1007/978-1-61779-564-0_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-563-3, 978-1-61779-564-0
|
| Authors |
Gibson DG, Daniel G. Gibson, Gibson, Daniel G. |
| Abstract |
The yeast Saccharomyces cerevisiae can take up and assemble at least 38 overlapping single-stranded oligonucleotides and a linear double-stranded vector in one transformation event. These oligonucleotides can overlap by as few as 20 bp and can be as long as 200 nucleotides in length to produce kilobase-sized synthetic DNA molecules. A protocol for designing the oligonucleotides to be assembled, transforming them into yeast, and confirming their assembly is described here. This straightforward scheme for assembling chemically synthesized oligonucleotides can be a useful tool for building synthetic DNA molecules. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 33% |
| United States | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 2% |
| France | 1 | 2% |
| Australia | 1 | 2% |
| United Kingdom | 1 | 2% |
| United States | 1 | 2% |
| Unknown | 42 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 13 | 28% |
| Student > Master | 8 | 17% |
| Student > Ph. D. Student | 6 | 13% |
| Student > Bachelor | 5 | 11% |
| Other | 2 | 4% |
| Other | 8 | 17% |
| Unknown | 5 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 47% |
| Biochemistry, Genetics and Molecular Biology | 9 | 19% |
| Computer Science | 2 | 4% |
| Medicine and Dentistry | 2 | 4% |
| Immunology and Microbiology | 2 | 4% |
| Other | 3 | 6% |
| Unknown | 7 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 June 2012.
All research outputs
#13,666,300
of 22,668,244 outputs
Outputs from Methods in molecular biology
#3,819
of 13,025 outputs
Outputs of similar age
#151,387
of 250,724 outputs
Outputs of similar age from Methods in molecular biology
#235
of 461 outputs
Altmetric has tracked 22,668,244 research outputs across all sources so far. This one is in the 38th percentile – i.e., 38% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,025 research outputs from this source. They receive a mean Attention Score of 3.3. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 250,724 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 461 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.