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Chapter title |
Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS).
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Chapter number | 5 |
Book title |
The Nuclear Envelope
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3530-7_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3528-4, 978-1-4939-3530-7
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Authors |
Shimi Takeshi, Chan-Gi Pack, Robert D. Goldman, Takeshi, Shimi, Pack, Chan-Gi, Goldman, Robert D. |
Editors |
Sue Shackleton, Philippe Collas, Eric C. Schirmer |
Abstract |
The major structural components of the nuclear lamina are the A- and B-type nuclear lamin proteins which are also present in the nucleoplasm. Studies of molecular movements of the lamins in both the lamina and nucleoplasm of living cell nuclei have provided insights into their roles in maintaining nuclear architecture. In this chapter, we present protocols for quantitatively measuring the mobilities of lamin proteins by fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) in mammalian cell nuclei. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 2 | 14% |
Student > Bachelor | 2 | 14% |
Student > Ph. D. Student | 2 | 14% |
Researcher | 1 | 7% |
Student > Postgraduate | 1 | 7% |
Other | 0 | 0% |
Unknown | 6 | 43% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 29% |
Engineering | 2 | 14% |
Medicine and Dentistry | 1 | 7% |
Chemistry | 1 | 7% |
Unknown | 6 | 43% |