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The Nuclear Envelope

Overview of attention for book
Cover of 'The Nuclear Envelope'

Table of Contents

  1. Altmetric Badge
    Book Overview
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    Chapter 1 The Nuclear Envelope
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    Chapter 2 Exploring the Protein Composition of the Plant Nuclear Envelope.
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    Chapter 3 High-Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes.
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    Chapter 4 The Nuclear Envelope
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    Chapter 5 Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS).
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    Chapter 6 The Nuclear Envelope
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    Chapter 7 The Use of Two-Photon FRET-FLIM to Study Protein Interactions During Nuclear Envelope Fusion In Vivo and In Vitro.
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    Chapter 8 Identifying Protein-Protein Associations at the Nuclear Envelope with BioID.
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    Chapter 9 In Situ Detection of Interactions Between Nuclear Envelope Proteins and Partners.
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    Chapter 10 The Nuclear Envelope
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    Chapter 11 Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional Complementation.
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    Chapter 12 The Nuclear Envelope
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    Chapter 13 Identification and Validation of Putative Nesprin Variants.
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    Chapter 14 Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting.
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    Chapter 15 The Use of Polyacrylamide Hydrogels to Study the Effects of Matrix Stiffness on Nuclear Envelope Properties.
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    Chapter 16 Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling.
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    Chapter 17 The Nuclear Envelope
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    Chapter 18 Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells.
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    Chapter 19 The Nuclear Envelope
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    Chapter 20 The Nuclear Envelope
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    Chapter 21 The Nuclear Envelope
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    Chapter 22 The Nuclear Envelope
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    Chapter 23 The Nuclear Envelope
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    Chapter 24 The Nuclear Envelope
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    Chapter 25 The Nuclear Envelope
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    Chapter 26 Methods to Monitor DNA Repair Defects and Genomic Instability in the Context of a Disrupted Nuclear Lamina.
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    Chapter 27 The Nuclear Envelope
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    Chapter 28 An In Vitro Assay to Study Targeting of Membrane Proteins to the Inner Nuclear Membrane.
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    Chapter 29 Nuclear Protein Transport in Digitonin Permeabilized Cells.
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    Chapter 30 Analysis of CRM1-Dependent Nuclear Export in Permeabilized Cells.
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    Chapter 31 The Nuclear Envelope
Attention for Chapter 24: The Nuclear Envelope
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Chapter title
The Nuclear Envelope
Chapter number 24
Book title
The Nuclear Envelope
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3530-7_24
Pubmed ID
27147055
Book ISBNs
978-1-4939-3528-4, 978-1-4939-3530-7
Authors

Clements, Craig S, Bikkul, Ural, Ahmed, Mai Hassan, Foster, Helen A, Godwin, Lauren S, Bridger, Joanna M, Craig S. Clements, Ural Bikkul, Mai Hassan Ahmed, Helen A. Foster, Lauren S. Godwin, Joanna M. Bridger, Clements, Craig S., Foster, Helen A., Godwin, Lauren S., Bridger, Joanna M.

Editors

Sue Shackleton, Philippe Collas, Eric C. Schirmer

Abstract

The genome has a special relationship with the nuclear envelope in cells. Much of the genome is anchored at the nuclear periphery, tethered by chromatin binding proteins such nuclear lamins and other integral membrane proteins. Even though there are global assays such as DAM-ID or ChIP to assess what parts of the genome are associated with the nuclear envelope, it is also essential to be able to visualize regions of the genome in order to reveal their individual relationships with nuclear structures in single cells. This is executed by fluorescence in situ hybridization (FISH) in 2-dimensional flattened nuclei (2D-FISH) or 3-dimensionally preserved cells (3D-FISH) in combination with indirect immunofluorescence to reveal structural proteins. This chapter explains the protocols for 2D- and 3D-FISH in combination with indirect immunofluorescence and discusses options for image capture and analysis. Due to the nuclear envelope proteins being part of the non-extractable nucleoskeleton, we also describe how to prepare DNA halos through salt extraction and how they can be used to study genome behavior and association when combined with 2D-FISH.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 50%
Researcher 2 14%
Student > Doctoral Student 1 7%
Student > Bachelor 1 7%
Other 1 7%
Other 0 0%
Unknown 2 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 50%
Agricultural and Biological Sciences 3 21%
Computer Science 2 14%
Unknown 2 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2017.
All research outputs
#18,154,932
of 23,322,258 outputs
Outputs from Methods in molecular biology
#7,406
of 13,329 outputs
Outputs of similar age
#270,562
of 396,123 outputs
Outputs of similar age from Methods in molecular biology
#758
of 1,473 outputs
Altmetric has tracked 23,322,258 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,329 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 396,123 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,473 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.

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