Chapter title |
Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition
|
---|---|
Chapter number | 6 |
Book title |
Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3332-7_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3330-3, 978-1-4939-3332-7
|
Authors |
Pérez-Alonso, Naivy, Chong-Pérez, Borys, Capote, Alina, Pérez, Anabel, Gerth, André, Angenon, Geert, Jiménez, Elio, Naivy Pérez-Alonso, Borys Chong-Pérez, Alina Capote, Anabel Pérez, André Gerth, Geert Angenon, Elio Jiménez |
Abstract |
Digitalis purpurea L. is one of the main economically viable sources of cardenolides (cardiac glycosides) for the pharmaceutical industry. Nevertheless, production of cardenolides in plants grown by traditional agriculture is not always an efficient process and can be affected by biotic and abiotic factors. This chapter provides two biotechnology strategies for biomass and cardenolide production in D. purpurea. Firstly, we report biomass production using a temporary immersion system (TIS), combined with cardenolide extraction and quantification. Secondly, an efficient protocol for genetic transformation via Agrobacterium tumefaciens is provided. These strategies can be used independently or combined in order to increase the content of cardiac glycosides in D. purpurea and to unravel biosynthetic pathways associated to cardiac glycoside production. |
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Unknown | 2 | 100% |
Demographic breakdown
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 36% |
Professor | 2 | 14% |
Researcher | 2 | 14% |
Student > Master | 1 | 7% |
Student > Bachelor | 1 | 7% |
Other | 0 | 0% |
Unknown | 3 | 21% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 5 | 36% |
Agricultural and Biological Sciences | 3 | 21% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Unknown | 5 | 36% |