Chapter title |
In Vitro Callus Induction and Plant Regeneration from Stem Explants of Ceropegia noorjahaniae , a Critically Endangered Medicinal Herb
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Chapter number | 24 |
Book title |
Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3332-7_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3330-3, 978-1-4939-3332-7
|
Authors |
Jaykumar J. Chavan, Mahendra L. Ahire, Chavan, Jaykumar J., Ahire, Mahendra L. |
Abstract |
An efficient protocol has been developed for in vitro regeneration of a large number of plantlets of Ceropegia noorjahaniae Ansari via indirect organogenesis from stem explants excised from in vitro-germinated seedlings. The callus was efficiently induced from the stem explants using Murashige and Skoog (MS) medium supplemented with auxins and their combinations. The highest number of shoots (16.0 ± 0.2) and shoot length (5.5 ± 0.1 cm) was achieved when the callus was subcultured to MS medium supplemented with 6-benzylaminopurine, BAP (2.0 mg/l) and indole-3-acetic acid, IAA (0.2 mg/l). The in vitro-developed shoots were rooted well in half-strength MS medium supplemented with 1.0 mg/l of indole-3-butyric acid (IBA) and 0.3 mg/l of α-naphthalene acetic acid (NAA). The plantlets were successfully hardened with 82 % survival rate. This is the first report on the regeneration of plants through indirect shoot organogenesis from stem derived calli of C. noorjahaniae. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Researcher | 1 | 20% |
Lecturer | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 3 | 60% |
Environmental Science | 1 | 20% |
Unknown | 1 | 20% |