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Immunocytochemical Methods and Protocols

Overview of attention for book
Cover of 'Immunocytochemical Methods and Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Overview of Antibodies for Immunochemistry
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    Chapter 2 Introduction to the Purification of Antibodies
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    Chapter 3 Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration
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    Chapter 4 Antibody Purification: Ion-Exchange Chromatography
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    Chapter 5 Antibody Purification: Affinity Chromatography – Protein A and Protein G Sepharose
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    Chapter 6 Conjugation of Fluorochromes to Antibodies
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    Chapter 7 Biotinylation of Antibodies
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    Chapter 8 Cell Fixatives for Immunostaining
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    Chapter 9 Permeabilization of Cell Membranes
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    Chapter 10 Preparation of Frozen Sections for Analysis
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    Chapter 11 Processing of Cytological Specimens
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    Chapter 12 Processing of Tissue Culture Cells
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    Chapter 13 Processing of Tissue Specimens
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    Chapter 14 Heat-induced antigen retrieval for immunohistochemical reactions in routinely processed paraffin sections.
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    Chapter 15 Fluorochromes: Properties and Characteristics
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    Chapter 16 Direct Immunofluorescent Labeling of Cells
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    Chapter 17 Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells
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    Chapter 18 Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells
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    Chapter 19 Fluorescent Visualization of Macromolecules in Drosophila Whole Mounts
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    Chapter 20 Overview of Conventional Fluorescence Photomicrography
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    Chapter 21 Overview of Confocal Microscopy
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    Chapter 22 Overview of Laser Microbeam Applications as Related to Antibody Targeting
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    Chapter 23 Immuno-Laser Capture Microdissection of Rat Brain Neurons for Real Time Quantitative PCR
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    Chapter 24 Overview of Antigen Detection Through Enzymatic Activity
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    Chapter 25 The Peroxidase–Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods
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    Chapter 26 The Avidin–Biotin Complex (ABC) Method and Other Avidin–Biotin Binding Methods
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    Chapter 27 Avidin-biotin labeling of cellular antigens in cryostat-sectioned tissue.
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    Chapter 28 Multiple antigen immunostaining procedures.
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    Chapter 29 Immunoenzymatic Quantitative Analysis of Antigens Expressed on the Cell Surface (Cell-ELISA)
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    Chapter 30 Use of Immunogold with Silver Enhancement
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    Chapter 31 Overview of Flow Cytometry and Fluorescent Probes for Flow Cytometry
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    Chapter 32 Tissue Disaggregation
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    Chapter 33 Indirect Immunofluorescent Labeling of Viable Cells
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    Chapter 34 Indirect Immunofluorescent Labeling of Fixed Cells
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    Chapter 35 Fluorescent Labeling of DNA
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    Chapter 36 Deparaffinization and Processing of Pathologic Material
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    Chapter 37 Fixation and Embedding
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    Chapter 38 Preparation of colloidal gold.
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    Chapter 39 Conjugation of colloidal gold to proteins.
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    Chapter 40 Colloidal Gold/Streptavidin Methods
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    Chapter 41 Pre-embedding Labeling Methods
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    Chapter 42 Postembedding Labeling Methods
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    Chapter 43 The Clinical Immunohistochemistry Laboratory: Regulations and Troubleshooting Guidelines
Attention for Chapter 26: The Avidin–Biotin Complex (ABC) Method and Other Avidin–Biotin Binding Methods
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)
  • High Attention Score compared to outputs of the same age and source (97th percentile)

Mentioned by

1 news outlet
1 patent


49 Dimensions

Readers on

117 Mendeley
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Chapter title
The Avidin–Biotin Complex (ABC) Method and Other Avidin–Biotin Binding Methods
Chapter number 26
Book title
Immunocytochemical Methods and Protocols
Published in
Methods in molecular biology, October 2009
DOI 10.1007/978-1-59745-324-0_26
Pubmed ID
Book ISBNs
978-1-58829-463-0, 978-1-59745-324-0

Gary L. Bratthauer, Bratthauer, Gary L.


Constance Oliver, Maria Célia Jamur

Mendeley readers

The data shown below were compiled from readership statistics for 117 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 <1%
United States 1 <1%
Unknown 115 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 36 31%
Student > Master 20 17%
Student > Ph. D. Student 17 15%
Student > Doctoral Student 10 9%
Researcher 5 4%
Other 10 9%
Unknown 19 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 21%
Biochemistry, Genetics and Molecular Biology 23 20%
Medicine and Dentistry 15 13%
Neuroscience 10 9%
Engineering 5 4%
Other 21 18%
Unknown 19 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2020.
All research outputs
of 17,367,552 outputs
Outputs from Methods in molecular biology
of 9,919 outputs
Outputs of similar age
of 272,686 outputs
Outputs of similar age from Methods in molecular biology
of 34 outputs
Altmetric has tracked 17,367,552 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,919 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 272,686 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 34 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.