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Immunocytochemical Methods and Protocols

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Cover of 'Immunocytochemical Methods and Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Overview of Antibodies for Immunochemistry
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    Chapter 2 Introduction to the Purification of Antibodies
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    Chapter 3 Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration
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    Chapter 4 Antibody Purification: Ion-Exchange Chromatography
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    Chapter 5 Antibody Purification: Affinity Chromatography – Protein A and Protein G Sepharose
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    Chapter 6 Conjugation of Fluorochromes to Antibodies
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    Chapter 7 Biotinylation of Antibodies
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    Chapter 8 Cell Fixatives for Immunostaining
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    Chapter 9 Permeabilization of Cell Membranes
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    Chapter 10 Preparation of Frozen Sections for Analysis
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    Chapter 11 Processing of Cytological Specimens
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    Chapter 12 Processing of Tissue Culture Cells
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    Chapter 13 Processing of Tissue Specimens
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    Chapter 14 Heat-induced antigen retrieval for immunohistochemical reactions in routinely processed paraffin sections.
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    Chapter 15 Fluorochromes: Properties and Characteristics
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    Chapter 16 Direct Immunofluorescent Labeling of Cells
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    Chapter 17 Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells
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    Chapter 18 Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells
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    Chapter 19 Fluorescent Visualization of Macromolecules in Drosophila Whole Mounts
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    Chapter 20 Overview of Conventional Fluorescence Photomicrography
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    Chapter 21 Overview of Confocal Microscopy
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    Chapter 22 Overview of Laser Microbeam Applications as Related to Antibody Targeting
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    Chapter 23 Immuno-Laser Capture Microdissection of Rat Brain Neurons for Real Time Quantitative PCR
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    Chapter 24 Overview of Antigen Detection Through Enzymatic Activity
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    Chapter 25 The Peroxidase–Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods
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    Chapter 26 The Avidin–Biotin Complex (ABC) Method and Other Avidin–Biotin Binding Methods
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    Chapter 27 Avidin-biotin labeling of cellular antigens in cryostat-sectioned tissue.
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    Chapter 28 Multiple antigen immunostaining procedures.
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    Chapter 29 Immunoenzymatic Quantitative Analysis of Antigens Expressed on the Cell Surface (Cell-ELISA)
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    Chapter 30 Use of Immunogold with Silver Enhancement
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    Chapter 31 Overview of Flow Cytometry and Fluorescent Probes for Flow Cytometry
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    Chapter 32 Tissue Disaggregation
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    Chapter 33 Indirect Immunofluorescent Labeling of Viable Cells
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    Chapter 34 Indirect Immunofluorescent Labeling of Fixed Cells
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    Chapter 35 Fluorescent Labeling of DNA
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    Chapter 36 Deparaffinization and Processing of Pathologic Material
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    Chapter 37 Fixation and Embedding
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    Chapter 38 Preparation of colloidal gold.
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    Chapter 39 Conjugation of colloidal gold to proteins.
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    Chapter 40 Colloidal Gold/Streptavidin Methods
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    Chapter 41 Pre-embedding Labeling Methods
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    Chapter 42 Postembedding Labeling Methods
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    Chapter 43 The Clinical Immunohistochemistry Laboratory: Regulations and Troubleshooting Guidelines
Attention for Chapter 42: Postembedding Labeling Methods
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Chapter title
Postembedding Labeling Methods
Chapter number 42
Book title
Immunocytochemical Methods and Protocols
Published in
Methods in molecular biology, October 2009
DOI 10.1007/978-1-59745-324-0_42
Pubmed ID
Book ISBNs
978-1-58829-463-0, 978-1-59745-324-0
Authors

Constance Oliver

Editors

Constance Oliver, Maria Célia Jamur

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 5%
Unknown 20 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 24%
Student > Master 4 19%
Researcher 4 19%
Student > Bachelor 3 14%
Student > Doctoral Student 2 10%
Other 3 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 52%
Neuroscience 4 19%
Computer Science 2 10%
Psychology 2 10%
Medicine and Dentistry 1 5%
Other 1 5%