Chapter title |
DNA-Based Characterization and Identification of Arbuscular Mycorrhizal Fungi Species.
|
---|---|
Chapter number | 6 |
Book title |
Microbial Environmental Genomics (MEG)
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3369-3_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3367-9, 978-1-4939-3369-3
|
Authors |
Senés-Guerrero, Carolina, Schüßler, Arthur, Carolina Senés-Guerrero, Arthur Schüßler |
Editors |
Francis Martin, Stephane Uroz |
Abstract |
Arbuscular mycorrhizal fungi (AMF) are obligate symbionts of most land plants. They have great ecological and economic importance as they can improve plant nutrition, plant water supply, soil structure, and plant resistance to pathogens. We describe two approaches for the DNA-based characterization and identification of AMF, which both can be used for single fungal spores, soil, or roots samples and resolve closely related AMF species: (a) Sanger sequencing of a 1.5 kb extended rDNA-barcode from clone libraries, e.g., to characterize AMF isolates, and (b) high throughput 454 GS-FLX+ pyrosequencing of a 0.8 kb rDNA fragment, e.g., for in-field monitoring. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 19% |
Researcher | 5 | 19% |
Student > Doctoral Student | 4 | 15% |
Student > Bachelor | 2 | 7% |
Student > Master | 2 | 7% |
Other | 2 | 7% |
Unknown | 7 | 26% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 16 | 59% |
Biochemistry, Genetics and Molecular Biology | 2 | 7% |
Environmental Science | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Unknown | 7 | 26% |