Chapter title |
Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening
|
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Chapter number | 2 |
Book title |
Kinase Screening and Profiling
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3073-9_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3072-2, 978-1-4939-3073-9
|
Authors |
Clint D. J. Tavares, Ashwini K. Devkota, Kevin N. Dalby, Eun Jeong Cho |
Abstract |
Protein kinases have emerged as an important class of therapeutic targets, as they are known to be involved in pathological pathways linked to numerous human disorders. Major efforts to discover kinase inhibitors in both academia and pharmaceutical companies have centered on the development of robust assays and cost-effective approaches to isolate them. Drug discovery procedures often start with hit identification for lead development, by screening a library of chemicals using an appropriate assay in a high-throughput manner. Considering limitations unique to each assay technique and screening capability, intelligent integration of various assay schemes and level of throughput, in addition to the choice of chemical libraries, is the key to success of this initial step. Here, we describe the purification of the protein kinase, eEF-2K, and the utilization of three biochemical assays in the course of identifying small molecules that block its enzymatic reaction. |
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Geographical breakdown
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Spain | 1 | 14% |
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Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 43% |
Unspecified | 1 | 14% |
Professor > Associate Professor | 1 | 14% |
Student > Master | 1 | 14% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
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Unspecified | 1 | 14% |
Computer Science | 1 | 14% |
Psychology | 1 | 14% |
Other | 0 | 0% |
Unknown | 1 | 14% |