Chapter title |
A High-Throughput Radiometric Kinase Assay
|
---|---|
Chapter number | 7 |
Book title |
Kinase Screening and Profiling
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3073-9_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3072-2, 978-1-4939-3073-9
|
Authors |
Krisna C. Duong-Ly, Jeffrey R. Peterson |
Abstract |
Aberrant kinase signaling has been implicated in a number of diseases. While kinases have become attractive drug targets, only a small fraction of human protein kinases have validated inhibitors. Screening of libraries of compounds against a kinase or kinases of interest is routinely performed during kinase inhibitor development to identify promising scaffolds for a particular target and to identify kinase targets for compounds of interest. Screening of more focused compound libraries may also be conducted in the later stages of inhibitor development to improve potency and optimize selectivity. The dot blot kinase assay is a robust, high-throughput kinase assay that can be used to screen a number of small-molecule compounds against one kinase of interest or several kinases. Here, a protocol for a dot blot kinase assay used for measuring insulin receptor kinase activity is presented. This protocol can be readily adapted for use with other protein kinases. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 6 | 33% |
Student > Bachelor | 4 | 22% |
Student > Ph. D. Student | 3 | 17% |
Professor | 1 | 6% |
Student > Doctoral Student | 1 | 6% |
Other | 1 | 6% |
Unknown | 2 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 44% |
Chemistry | 4 | 22% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 11% |
Agricultural and Biological Sciences | 1 | 6% |
Unknown | 3 | 17% |