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Mendeley readers
Chapter title |
Creating Knockout and Knockin Rodents Using Engineered Endonucleases via Direct Embryo Injection.
|
---|---|
Chapter number | 18 |
Book title |
Chromosomal Mutagenesis
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-1862-1_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1861-4, 978-1-4939-1862-1
|
Authors |
Takehito Kaneko, Tomoji Mashimo |
Abstract |
Genetically engineered rodents have been generated worldwide for biomedical research. Recently, gene targeting techniques have been developed by using engineered endonucleases such as zinc-finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. These endonucleases are useful for simple and rapid production of gene knockout/knockin animals without using embryonic stem (ES) cells. This chapter introduces the latest protocols for producing genetically modified rodents using ZFN, TALEN, and CRISPR/Cas9. |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 3% |
India | 1 | 3% |
Unknown | 31 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 15% |
Student > Bachelor | 5 | 15% |
Researcher | 4 | 12% |
Student > Master | 4 | 12% |
Student > Doctoral Student | 2 | 6% |
Other | 3 | 9% |
Unknown | 10 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 8 | 24% |
Biochemistry, Genetics and Molecular Biology | 8 | 24% |
Medicine and Dentistry | 4 | 12% |
Unspecified | 1 | 3% |
Social Sciences | 1 | 3% |
Other | 1 | 3% |
Unknown | 10 | 30% |