| Chapter title |
Genome Engineering Using Adeno-Associated Virus (AAV).
|
|---|---|
| Chapter number | 5 |
| Book title |
Chromosomal Mutagenesis
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-1862-1_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-1861-4, 978-1-4939-1862-1
|
| Authors |
Rob Howes, Christine Schofield, Howes, Rob, Schofield, Christine |
| Abstract |
The ability to edit the genome of cell lines has provided valuable insights into biological processes and the contribution of specific mutations to disease biology. These techniques fall into two categories based on the DNA repair mechanism that is used to incorporate the genetic change. Nuclease-based technologies, such as Zinc-Finger Nucleases, TALENS, and Crispr/Cas9, rely on non-homologous end-joining (NHEJ) and homology directed repair (HDR) to generate a range of genetic modifications. Adeno-Associated Virus (AAV) utilizes homologous recombination to generate precise and predictable genetic modifications directly at the target locus. AAV has been used to create over 500 human isogenic cell lines comprising a wide range of genetic alterations from gene knockouts, insertions of point mutations, indels, epitope tags, and reporter genes. Here we describe the generation and use of AAV gene targeting vectors and viruses to create targeted isogenic cell lines. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 4% |
| Unknown | 27 | 96% |
Demographic breakdown
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|---|---|---|
| Researcher | 8 | 29% |
| Student > Ph. D. Student | 7 | 25% |
| Student > Bachelor | 3 | 11% |
| Professor | 2 | 7% |
| Student > Master | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 4 | 14% |
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|---|---|---|
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| Biochemistry, Genetics and Molecular Biology | 3 | 11% |
| Immunology and Microbiology | 2 | 7% |
| Engineering | 2 | 7% |
| Other | 5 | 18% |
| Unknown | 5 | 18% |