Chapter title |
SRYTH: A New Yeast Two-Hybrid Method.
|
---|---|
Chapter number | 3 |
Book title |
Candida Species
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3052-4_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3051-7, 978-1-4939-3052-4
|
Authors |
Mallick, Jaideep, Jansen, Gregor, Wu, Cunle, Whiteway, Malcolm, Jaideep Mallick, Gregor Jansen, Cunle Wu, Malcolm Whiteway |
Abstract |
Many biological processes are regulated by protein-protein interactions, and the analysis of these interactions has been a productive endeavor contributing to our understanding of cellular organization and function. The yeast two-hybrid technique is a widely used, powerful method of analyzing protein-protein interactions. The currently used formats, however, have inherent limitations, providing an opportunity to develop new alternatives that extend our ability to detect protein-protein interactions of biological relevance. Here we present a two-hybrid system named SRYTH (Ste11p/Ste50p related yeast two-hybrid) based on the Ste11p/Ste50p interaction that uses the activation of the HOG pathway of Saccharomyces cerevisiae as a reporter for interactions. The system is suitable for detecting cytoplasmic protein interactions in their natural subcellular environment, and has been successfully used to investigate protein-protein interactions, including transcription factor associations, in Candida albicans. |
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