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Candida Species

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Attention for Chapter 4: Chromatin Immunoprecipitation (ChIP) Assay in Candida albicans.
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Chapter title
Chromatin Immunoprecipitation (ChIP) Assay in Candida albicans.
Chapter number 4
Book title
Candida Species
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3052-4_4
Pubmed ID
Book ISBNs
978-1-4939-3051-7, 978-1-4939-3052-4
Authors

Mitra, Sreyoshi, Rai, Laxmi Shanker, Chatterjee, Gautam, Sanyal, Kaustuv, Sreyoshi Mitra, Laxmi Shanker Rai, Gautam Chatterjee, Kaustuv Sanyal

Abstract

Chromatin immunoprecipitation (ChIP) is a widely used technique which can determine the in vivo association of a specific protein on a particular DNA locus in the genome. In this method cross-linked chromatin is sheared and immunoprecipitated with antibodies raised against a target protein of interest. The end result of this process is the enrichment of DNA fragments associated with the desired protein. Thus, interactions between proteins and genomic loci in cellular context can be determined by this technique. Here, we are describing a ChIP protocol that is optimized for Candida albicans. The protocol requires 4-5 days for completion of the assay and has been used to produce robust ChIP results for diverse proteins in this organism and its related species including Candida dubliniensis and Candida tropicalis.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 25%
Other 1 13%
Student > Ph. D. Student 1 13%
Researcher 1 13%
Student > Postgraduate 1 13%
Other 0 0%
Unknown 2 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 38%
Immunology and Microbiology 2 25%
Computer Science 1 13%
Unknown 2 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 May 2016.
All research outputs
#18,429,829
of 22,831,537 outputs
Outputs from Methods in molecular biology
#7,920
of 13,126 outputs
Outputs of similar age
#284,422
of 393,555 outputs
Outputs of similar age from Methods in molecular biology
#846
of 1,470 outputs
Altmetric has tracked 22,831,537 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,126 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.