Estrogen Receptors
Humana Press
Chapter title |
Expression Profiles of Estrogen-Regulated MicroRNAs in Breast Cancer Cells.
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Chapter number | 30 |
Book title |
Estrogen Receptors
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3127-9_30 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3126-2, 978-1-4939-3127-9
|
Authors |
Katchy, Anne, Williams, Cecilia, Anne Katchy, Cecilia Williams |
Abstract |
Molecular signaling through both estrogen and microRNAs are critical for breast cancer development and growth. The activity of estrogen is mediated by transcription factors, the estrogen receptors. Here we describe a method for robust characterization of estrogen-regulated microRNA profiles. The method details how to prepare cells for optimal estrogen response, directions for estrogen treatment, RNA extraction, microRNA large-scale profiling, and subsequent confirmations. |
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Student > Ph. D. Student | 3 | 12% |
Professor | 2 | 8% |
Researcher | 2 | 8% |
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