Chapter title |
Analysis of G-Protein Coupled Receptor 30 (GPR30) on Endothelial Inflammation
|
---|---|
Chapter number | 39 |
Book title |
Estrogen Receptors
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3127-9_39 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3126-2, 978-1-4939-3127-9
|
Authors |
Subhadeep Chakrabarti, Sandra T. Davidge, Chakrabarti, Subhadeep, Davidge, Sandra T. |
Abstract |
The female sex hormone estrogen (the most common form 17-β-estradiol or E2) is known to have both anti-inflammatory and pro-inflammatory effects. Given the diversity of estrogen responses mediated through its three distinct receptors, namely, estrogen receptor α (ERα), ERβ, and the G-protein coupled receptor 30 (GPR30), it is plausible that different receptors have specific modulatory effects on inflammation in different tissues. We have shown that activation of GPR30 exerted anti-inflammatory effects as demonstrated by significant attenuation of tumor necrosis factor (TNF)-mediated upregulation of adhesion molecules in isolated human umbilical vein endothelial cells. Interestingly, estrogen alone had no such effect and blockade of classical ERs restored the anti-inflammatory effect, suggesting that this effect was dependent on GPR30 and opposed to classical ERs. These findings were further validated by the negation of anti-inflammatory GPR30 effects by classical ER agonists. This chapter focuses on multiple pharmacological options to activate GPR30 and the use of TNF activated endothelial cells as a model system for inflammatory response as assessed by adhesion molecule detection through western blotting. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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New Zealand | 1 | 14% |
Unknown | 6 | 86% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 2 | 29% |
Professor > Associate Professor | 1 | 14% |
Researcher | 1 | 14% |
Unknown | 3 | 43% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 2 | 29% |
Agricultural and Biological Sciences | 1 | 14% |
Nursing and Health Professions | 1 | 14% |
Unknown | 3 | 43% |