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Chapter title |
Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen
|
---|---|
Chapter number | 5 |
Book title |
Estrogen Receptors
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3127-9_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3126-2, 978-1-4939-3127-9
|
Authors |
Kwi Hye Koh, Hyunyoung Jeong, Koh, Kwi Hye, Jeong, Hyunyoung |
Abstract |
Electrophoretic mobility shift assay (EMSA) is an invaluable tool to study interaction of proteins with DNA. Estrogens are major female hormones and modulate biological function through estrogen receptor (ER). ER regulates its target gene expression via the classical mechanism in which ER directly binds to its target gene promoter or the nonclassical mechanism involving tethering of ER to other transcription factors (such as AP-1 proteins). Here, we describe the EMSA to examine the nonclassical mechanism of ER action in regulation of a gene CYP2B6 by using competition and supershift assays. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 29% |
Student > Bachelor | 1 | 14% |
Student > Master | 1 | 14% |
Researcher | 1 | 14% |
Professor > Associate Professor | 1 | 14% |
Other | 0 | 0% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 29% |
Biochemistry, Genetics and Molecular Biology | 1 | 14% |
Computer Science | 1 | 14% |
Immunology and Microbiology | 1 | 14% |
Neuroscience | 1 | 14% |
Other | 0 | 0% |
Unknown | 1 | 14% |