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Mendeley readers
| Chapter title |
Functional Analysis of the Role of Toxin-Antitoxin (TA) Loci in Bacterial Persistence.
|
|---|---|
| Chapter number | 11 |
| Book title |
Bacterial Persistence
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2854-5_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2853-8, 978-1-4939-2854-5
|
| Authors |
Butt, Aaron T, Titball, Richard W, Butt, Aaron T., Titball, Richard W., Aaron T. Butt, Richard W. Titball |
| Abstract |
We have developed a method to analyze the functionality of putative TA loci by expressing them in Escherichia coli. Here, we describe the procedure for cloning recombinant TA genes into inducible plasmids and expressing these in E. coli. Following expression, toxicity, resuscitation of growth, and changes in persister cell formation are assayed. This can confirm whether predicted TA loci are active in E. coli and whether expression can affect persister cell formation. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 33% |
| Student > Bachelor | 3 | 33% |
| Unspecified | 1 | 11% |
| Student > Postgraduate | 1 | 11% |
| Student > Master | 1 | 11% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 44% |
| Biochemistry, Genetics and Molecular Biology | 3 | 33% |
| Computer Science | 1 | 11% |
| Unspecified | 1 | 11% |