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Platelets and Megakaryocytes

Overview of attention for book
Cover of 'Platelets and Megakaryocytes'

Table of Contents

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    Book Overview
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    Chapter 1 Immobilization of Nonactivated Unfixed Platelets for Real-Time Single-Cell Analysis
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    Chapter 2 Imaging Platelets and Megakaryocytes by High-Resolution Laser Fluorescence Microscopy
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    Chapter 3 Single-Molecule Localization and Structured Illumination Microscopy of Platelet Proteins
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    Chapter 4 Electron Tomography and Correlative Approaches in Platelet Studies
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    Chapter 5 Screening and High-Throughput Platelet Assays
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    Chapter 6 High-Throughput Signaling Profiling in Blood Platelets by Multiplexed Phosphoflow Cytometry
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    Chapter 7 Precise Quantification of Platelet Proteins and Their Phosphorylation States
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    Chapter 8 The Study of Platelet Receptors Using Artificial Lipid Bilayers
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    Chapter 9 Three-Dimensional Culture in a Methylcellulose-Based Hydrogel to Study the Impact of Stiffness on Megakaryocyte Differentiation
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    Chapter 10 Differentiation of Human Pluripotent Stem Cells to Megakaryocytes by Transcription Factor-Driven Forward Programming
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    Chapter 11 Three-Dimensional Tissue Models for Studying Ex Vivo Megakaryocytopoiesis and Platelet Production
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    Chapter 12 Fluorescence Approaches to Image and Quantify the Demarcation Membrane System in Living Megakaryocytes
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    Chapter 13 High-Resolution 3D Imaging of Megakaryocytes Using Focused Ion Beam-Scanning Electron Microscopy
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    Chapter 14 Optical Clearing of Murine Bones to Study Megakaryocytes in Intact Bone Marrow Using Light-Sheet Fluorescence Microscopy
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    Chapter 15 Mathematical Techniques for Understanding Platelet Regulation and the Development of New Pharmacological Approaches
Attention for Chapter 11: Three-Dimensional Tissue Models for Studying Ex Vivo Megakaryocytopoiesis and Platelet Production
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Chapter title
Three-Dimensional Tissue Models for Studying Ex Vivo Megakaryocytopoiesis and Platelet Production
Chapter number 11
Book title
Platelets and Megakaryocytes
Published in
Methods in molecular biology, September 2018
DOI 10.1007/978-1-4939-8585-2_11
Pubmed ID
30171579
Book ISBNs
978-1-4939-8584-5, 978-1-4939-8585-2
Authors

Christian A. Di Buduo, Vittorio Abbonante, Lorenzo Tozzi, David L. Kaplan, Alessandra Balduini, Di Buduo, Christian A., Abbonante, Vittorio, Tozzi, Lorenzo, Kaplan, David L., Balduini, Alessandra

Abstract

Three-dimensional (3D) tissue cultures in vitro enable a more physiological reconstruction of native tissues and organs. The bone marrow environment, structure and composition regulate megakaryocyte function and platelet production. Here, we describe the use of silk fibroin protein biomaterials to assemble 3D scaffolds mimicking the bone marrow niche architecture and extracellular matrix composition to support platelet release from human megakaryocytes. Additionally, we also propose the use of hyaluronan hydrogels, functionalized with extracellular matrix components, to reproduce the 3D matrix structure of the bone marrow environment for studying human megakaryocyte function.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 25%
Student > Doctoral Student 1 13%
Other 1 13%
Student > Bachelor 1 13%
Researcher 1 13%
Other 0 0%
Unknown 2 25%
Readers by discipline Count As %
Medicine and Dentistry 2 25%
Agricultural and Biological Sciences 2 25%
Biochemistry, Genetics and Molecular Biology 1 13%
Unknown 3 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 March 2019.
All research outputs
#20,532,290
of 23,102,082 outputs
Outputs from Methods in molecular biology
#9,977
of 13,208 outputs
Outputs of similar age
#292,456
of 335,775 outputs
Outputs of similar age from Methods in molecular biology
#188
of 248 outputs
Altmetric has tracked 23,102,082 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,208 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 248 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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