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Platelets and Megakaryocytes

Overview of attention for book
Cover of 'Platelets and Megakaryocytes'

Table of Contents

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    Book Overview
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    Chapter 1 Immobilization of Nonactivated Unfixed Platelets for Real-Time Single-Cell Analysis
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    Chapter 2 Imaging Platelets and Megakaryocytes by High-Resolution Laser Fluorescence Microscopy
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    Chapter 3 Single-Molecule Localization and Structured Illumination Microscopy of Platelet Proteins
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    Chapter 4 Electron Tomography and Correlative Approaches in Platelet Studies
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    Chapter 5 Screening and High-Throughput Platelet Assays
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    Chapter 6 High-Throughput Signaling Profiling in Blood Platelets by Multiplexed Phosphoflow Cytometry
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    Chapter 7 Precise Quantification of Platelet Proteins and Their Phosphorylation States
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    Chapter 8 The Study of Platelet Receptors Using Artificial Lipid Bilayers
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    Chapter 9 Three-Dimensional Culture in a Methylcellulose-Based Hydrogel to Study the Impact of Stiffness on Megakaryocyte Differentiation
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    Chapter 10 Differentiation of Human Pluripotent Stem Cells to Megakaryocytes by Transcription Factor-Driven Forward Programming
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    Chapter 11 Three-Dimensional Tissue Models for Studying Ex Vivo Megakaryocytopoiesis and Platelet Production
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    Chapter 12 Fluorescence Approaches to Image and Quantify the Demarcation Membrane System in Living Megakaryocytes
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    Chapter 13 High-Resolution 3D Imaging of Megakaryocytes Using Focused Ion Beam-Scanning Electron Microscopy
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    Chapter 14 Optical Clearing of Murine Bones to Study Megakaryocytes in Intact Bone Marrow Using Light-Sheet Fluorescence Microscopy
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    Chapter 15 Mathematical Techniques for Understanding Platelet Regulation and the Development of New Pharmacological Approaches
Attention for Chapter 14: Optical Clearing of Murine Bones to Study Megakaryocytes in Intact Bone Marrow Using Light-Sheet Fluorescence Microscopy
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Chapter title
Optical Clearing of Murine Bones to Study Megakaryocytes in Intact Bone Marrow Using Light-Sheet Fluorescence Microscopy
Chapter number 14
Book title
Platelets and Megakaryocytes
Published in
Methods in molecular biology, September 2018
DOI 10.1007/978-1-4939-8585-2_14
Pubmed ID
Book ISBNs
978-1-4939-8584-5, 978-1-4939-8585-2
Authors

Maximilian G. Gorelashvili, Katrin G. Heinze, David Stegner

Abstract

In mammals, the differentiation and maturation of megakaryocytes (MKs) occurs in the bone marrow (BM). The three-dimensional environment influences megakaryopoiesis and platelet release. Thus, imaging MKs within the intact BM is important to understand megakaryopoiesis. Here, we present an optical clearing protocol for intact bones and the subsequent microscopic analysis including image processing to quantitatively assess MK size and distribution. This technique overcomes the limitations of classical sectioning methods as the entire bone can be imaged.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 43%
Student > Master 1 14%
Unknown 3 43%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 14%
Agricultural and Biological Sciences 1 14%
Energy 1 14%
Neuroscience 1 14%
Unknown 3 43%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 September 2018.
All research outputs
#14,424,488
of 23,102,082 outputs
Outputs from Methods in molecular biology
#4,242
of 13,208 outputs
Outputs of similar age
#188,564
of 335,775 outputs
Outputs of similar age from Methods in molecular biology
#76
of 248 outputs
Altmetric has tracked 23,102,082 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,208 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 335,775 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 248 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.