Chapter title |
The Study of Platelet Receptors Using Artificial Lipid Bilayers
|
---|---|
Chapter number | 8 |
Book title |
Platelets and Megakaryocytes
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8585-2_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8584-5, 978-1-4939-8585-2
|
Authors |
Michael L. Dustin, Alice Y. Pollitt |
Abstract |
Artificial lipid bilayers are powerful tools that can be used to model the interactions between platelets and membrane-bound ligands. To mimic the interaction of platelets with membrane-bound ligands, biotinylated lipids can be used to couple monobiotinylated recombinant ligands to the upper leaflet of an artificial lipid bilayer using streptavidin to bridge the two. Artificial lipid bilayers are generated by preparing liposomes, treating glass coverslips to make them hydrophilic and by assembling the bilayer in a specialized flow chamber. Finally platelets can be added to the flow chamber and the localization of fluorescently labeled molecules followed using microscopy. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 33% |
Other | 1 | 33% |
Student > Master | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Agricultural and Biological Sciences | 1 | 33% |
Immunology and Microbiology | 1 | 33% |