Chapter title |
Hard-Soft Tissue Interface Engineering
|
---|---|
Chapter number | 14 |
Book title |
Engineering Mineralized and Load Bearing Tissues
|
Published in |
Advances in experimental medicine and biology, January 2015
|
DOI | 10.1007/978-3-319-22345-2_14 |
Pubmed ID | |
Book ISBNs |
978-3-31-922344-5, 978-3-31-922345-2
|
Authors |
Oshima, Masamitsu, Tsuji, Takashi, Masamitsu Oshima, Takashi Tsuji |
Abstract |
Dental problems caused by dental caries, periodontal disease and tooth injury compromise the oral and general health issues. Current advances for the development of regenerative therapy have been influenced by our understanding of embryonic development, stem cell biology, and tissue engineering technology. Tooth regenerative therapy for tooth tissue repair and whole tooth replacement is currently expected a novel therapeutic concept with the full recovery of tooth physiological functions. Dental stem cells and cell-activating cytokines are thought to be candidate approach for tooth tissue regeneration because they have the potential to differentiate into tooth tissues in vitro and in vivo. Whole tooth replacement therapy is considered to be an attractive concept for next generation regenerative therapy as a form of bioengineered organ replacement. For realization of whole tooth regeneration, we have developed a novel three-dimensional cell manipulation method designated the "organ germ method". This method involves compartmentalisation of epithelial and mesenchymal cells at a high cell density to mimic multicellular assembly conditions and epithelial-mesenchymal interactions in organogenesis. The bioengineered tooth germ generates a structurally correct tooth in vitro, and erupted successfully with correct tooth structure when transplanted into the oral cavity. We have ectopically generated a bioengineered tooth unit composed of a mature tooth, periodontal ligament and alveolar bone, and that tooth unit was engrafted into an adult jawbone through bone integration. Bioengineered teeth were also able to perform physiological tooth functions such as mastication, periodontal ligament function and response to noxious stimuli. In this review, we describe recent findings and technologies underpinning whole tooth regenerative therapy. |
X Demographics
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United States | 1 | 25% |
Latvia | 1 | 25% |
Unknown | 2 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 75% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
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Spain | 1 | 1% |
Chile | 1 | 1% |
Unknown | 66 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 11 | 16% |
Student > Ph. D. Student | 10 | 15% |
Student > Master | 8 | 12% |
Researcher | 6 | 9% |
Other | 5 | 7% |
Other | 8 | 12% |
Unknown | 20 | 29% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 22 | 32% |
Agricultural and Biological Sciences | 8 | 12% |
Biochemistry, Genetics and Molecular Biology | 7 | 10% |
Engineering | 3 | 4% |
Materials Science | 2 | 3% |
Other | 5 | 7% |
Unknown | 21 | 31% |