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The LINC Complex

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Cover of 'The LINC Complex'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 EM Tomography of Meiotic LINC Complexes
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    Chapter 2 Recombinant Purification of the Periplasmic Portion of the LINC Complex
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    Chapter 3 Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis
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    Chapter 4 Analysis of Nesprin-2 Interaction with Its Binding Partners and Actin
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    Chapter 5 Interactions of Nesprin-4-Containing LINC Complexes in Outer Hair Cells Explored by BioID
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    Chapter 6 Using Nesprin Tension Sensors to Measure Force on the LINC Complex
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    Chapter 7 Analyzing Mechanotransduction Through the LINC Complex in Isolated Nuclei
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    Chapter 8 Direct Force Probe for Nuclear Mechanics
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    Chapter 9 Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex-Dependent Mechanisms of Homeostatic Nuclear Positioning
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    Chapter 10 Assembly and Use of a Microfluidic Device to Study Cell Migration in Confined Environments
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    Chapter 11 Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy
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    Chapter 12 Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super-Resolution Imaging
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    Chapter 13 Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes During Development of Caenorhabditis elegans
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    Chapter 14 High-Resolution Imaging Methods to Analyze LINC Complex Function During Drosophila Muscle Development
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    Chapter 15 Computational Methods for Studying the Plant Nucleus
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    Chapter 16 Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System
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    Chapter 17 Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components
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    Chapter 18 Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice
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    Chapter 19 An In Vitro System to Measure the Positioning, Stiffness, and Rupture of the Nucleus in Skeletal Muscle
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    Chapter 20 Functional Analysis of LINC Complexes in the Skin
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    Chapter 21 Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer
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    Chapter 22 Next-Generation Sequencing and Mutational Analysis: Implications for Genes Encoding LINC Complex Proteins
Attention for Chapter 1: EM Tomography of Meiotic LINC Complexes
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Chapter title
EM Tomography of Meiotic LINC Complexes
Chapter number 1
Book title
The LINC Complex
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8691-0_1
Pubmed ID
Book ISBNs
978-1-4939-8690-3, 978-1-4939-8691-0

Spindler, Marie-Christin, Helmprobst, Frederik, Stigloher, Christian, Benavente, Ricardo, Marie-Christin Spindler, Frederik Helmprobst, Christian Stigloher, Ricardo Benavente


Electron microscope (EM) tomography is a powerful technique that enables the three-dimensional analysis of subcellular structures at high resolution. We have applied this method to the quantitative analysis of LINC complex distribution and interaction with the cytoskeleton in meiotic cells from male mice. In this chapter, we describe methods to generate and analyze the tomograms.

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Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 1 100%
Readers by discipline Count As %
Medicine and Dentistry 1 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 August 2018.
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