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Yeast Cytokinesis

Overview of attention for book
Cover of 'Yeast Cytokinesis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Time-Lapse Fluorescence Microscopy of Budding Yeast Cells.
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    Chapter 2 Real-Time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells.
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    Chapter 3 Fluorescence Recovery After Photo-Bleaching (FRAP) and Fluorescence Loss in Photo-Bleaching (FLIP) Experiments to Study Protein Dynamics During Budding Yeast Cell Division.
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    Chapter 4 High-Speed Super-Resolution Imaging of Live Fission Yeast Cells.
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    Chapter 5 Monitoring Chitin Deposition During Septum Assembly in Budding Yeast.
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    Chapter 6 Imaging Septum Formation by Fluorescence Microscopy.
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    Chapter 7 Visualization of Cytokinesis Events in Budding Yeast by Transmission Electron Microscopy.
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    Chapter 8 Visualization of Fission Yeast Cells by Transmission Electron Microscopy.
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    Chapter 9 Characterization of Septin Ultrastructure in Budding Yeast Using Electron Tomography.
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    Chapter 10 Isolation of Cytokinetic Actomyosin Rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe.
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    Chapter 11 Measurements of Myosin-II Motor Activity During Cytokinesis in Fission Yeast.
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    Chapter 12 In Vitro Biochemical Characterization of Cytokinesis Actin-Binding Proteins
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    Chapter 13 Characterization of Cytokinetic F-BARs and Other Membrane-Binding Proteins.
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    Chapter 14 Analysis of Three-Dimensional Structures of Exocyst Components.
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    Chapter 15 Analysis of Rho-GTPase Activity During Budding Yeast Cytokinesis.
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    Chapter 16 Detection of Phosphorylation Status of Cytokinetic Components.
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    Chapter 17 Studying Protein-Protein Interactions in Budding Yeast Using Co-immunoprecipitation.
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    Chapter 18 Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes.
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    Chapter 19 Synchronization of the Budding Yeast Saccharomyces cerevisiae.
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    Chapter 20 Fission Yeast Cell Cycle Synchronization Methods.
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    Chapter 21 A Review of Fluorescent Proteins for Use in Yeast.
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    Chapter 22 Visualization and Image Analysis of Yeast Cells.
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    Chapter 23 Toolbox for Protein Structure Prediction
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    Chapter 24 From Structure to Function: A Comprehensive Compendium of Tools to Unveil Protein Domains and Understand Their Role in Cytokinesis.
Attention for Chapter 8: Visualization of Fission Yeast Cells by Transmission Electron Microscopy.
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Chapter title
Visualization of Fission Yeast Cells by Transmission Electron Microscopy.
Chapter number 8
Book title
Yeast Cytokinesis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3145-3_8
Pubmed ID
26519308
Book ISBNs
978-1-4939-3144-6, 978-1-4939-3145-3
Authors

Sipiczki, Matthias, Matthias Sipiczki

Editors

Alberto Sanchez-Diaz, Pilar Perez

Abstract

This chapter deals with the preparation of fission yeast (Schizosaccharomyces) cells for ultrastructural examination. The structure of the cell must be preserved as close to the in vivo situation as possible. This can be achieved by either chemical or cryofixation; the latter will not be dealt with in this chapter. Aldehydes that cross-link proteins and permanganates that besides cross-linking also stain membranous and cell wall structures are used for chemical fixation. This step is followed by dehydration and embedding of the cells in epoxy or acrylic resin. Sectioning of the embedded material produces slices of the cells that have to be stained with heavy metals to increase contrast differences between different structures or can be used for immunodetection of antigens (polysaccharides or proteins) with specific primary antibodies and gold-conjugated secondary antibodies.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 25%
Researcher 1 25%
Student > Postgraduate 1 25%
Unknown 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 25%
Agricultural and Biological Sciences 1 25%
Medicine and Dentistry 1 25%
Unknown 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 November 2015.
All research outputs
#15,729,348
of 23,369,802 outputs
Outputs from Methods in molecular biology
#5,501
of 13,342 outputs
Outputs of similar age
#234,053
of 396,368 outputs
Outputs of similar age from Methods in molecular biology
#550
of 1,474 outputs
Altmetric has tracked 23,369,802 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,342 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 396,368 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,474 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.

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