Chapter title |
Synchronization of the Budding Yeast Saccharomyces cerevisiae.
|
---|---|
Chapter number | 19 |
Book title |
Yeast Cytokinesis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3145-3_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3144-6, 978-1-4939-3145-3
|
Authors |
Foltman, Magdalena, Molist, Iago, Sanchez-Diaz, Alberto, Magdalena Foltman, Iago Molist, Alberto Sanchez-Diaz |
Editors |
Alberto Sanchez-Diaz, Pilar Perez |
Abstract |
A number of model organisms have provided the basis for our understanding of the eukaryotic cell cycle. These model organisms are generally much easier to manipulate than mammalian cells and as such provide amenable tools for extensive genetic and biochemical analysis. One of the most common model organisms used to study the cell cycle is the budding yeast Saccharomyces cerevisiae. This model provides the ability to synchronise cells efficiently at different stages of the cell cycle, which in turn opens up the possibility for extensive and detailed study of mechanisms regulating the eukaryotic cell cycle. Here, we describe methods in which budding yeast cells are arrested at a particular phase of the cell cycle and then released from the block, permitting the study of molecular mechanisms that drive the progression through the cell cycle. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 6 | 30% |
Student > Ph. D. Student | 4 | 20% |
Researcher | 4 | 20% |
Student > Doctoral Student | 2 | 10% |
Lecturer | 1 | 5% |
Other | 1 | 5% |
Unknown | 2 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 45% |
Agricultural and Biological Sciences | 6 | 30% |
Chemical Engineering | 1 | 5% |
Engineering | 1 | 5% |
Unknown | 3 | 15% |