Chapter title |
Chromatin Immunoprecipitation (ChIP) Assay in Candida albicans.
|
---|---|
Chapter number | 4 |
Book title |
Candida Species
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3052-4_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3051-7, 978-1-4939-3052-4
|
Authors |
Mitra, Sreyoshi, Rai, Laxmi Shanker, Chatterjee, Gautam, Sanyal, Kaustuv, Sreyoshi Mitra, Laxmi Shanker Rai, Gautam Chatterjee, Kaustuv Sanyal |
Abstract |
Chromatin immunoprecipitation (ChIP) is a widely used technique which can determine the in vivo association of a specific protein on a particular DNA locus in the genome. In this method cross-linked chromatin is sheared and immunoprecipitated with antibodies raised against a target protein of interest. The end result of this process is the enrichment of DNA fragments associated with the desired protein. Thus, interactions between proteins and genomic loci in cellular context can be determined by this technique. Here, we are describing a ChIP protocol that is optimized for Candida albicans. The protocol requires 4-5 days for completion of the assay and has been used to produce robust ChIP results for diverse proteins in this organism and its related species including Candida dubliniensis and Candida tropicalis. |
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