Report for: Clathrin-Mediated Endoytosis

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Chapter title	Acute Manipulations of Clathrin-Mediated Endocytosis at Presynaptic Nerve Terminals
Chapter number	6
Book title	Clathrin-Mediated Endoytosis
Published in	Methods in molecular biology, August 2018
DOI	10.1007/978-1-4939-8719-1_6
Pubmed ID	30129010
Book ISBNs	978-1-4939-8717-7, 978-1-4939-8719-1
Authors	Rylie B. Walsh, Ona E. Bloom, Jennifer R. Morgan, Walsh, Rylie B., Bloom, Ona E., Morgan, Jennifer R.
Abstract	Acute perturbations of clathrin and associated proteins at synapses have provided a wealth of knowledge on the molecular mechanisms underlying clathrin-mediated endocytosis (CME). The basic approach entails presynaptic microinjection of an inhibitory reagent targeted to the CME pathway, followed by a detailed ultrastructural analysis to identify how the perturbation affects the number and distribution of synaptic vesicles, plasma membrane, clathrin-coated pits, and clathrin-coated vesicles. This chapter describes the methodology for acutely perturbing CME at the lamprey giant reticulospinal synapse, a model vertebrate synapse that has been instrumental for identifying key protein-protein interactions that regulate CME in presynaptic nerve terminals with broader extension to nonneuronal cell types.

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Unknown	1	100%

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Type	Count	As %
Members of the public	1	100%

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Professor	2	50%
Unknown	2	50%

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Neuroscience	2	50%
Unknown	2	50%

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This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2018.

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#15,543,612

of 23,100,534 outputs

Outputs from Methods in molecular biology

#5,412

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#211,310

of 333,760 outputs

Outputs of similar age from Methods in molecular biology

#97

of 249 outputs

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Clathrin-Mediated Endoytosis

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