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Mendeley readers
| Chapter title |
Measuring Clathrin-Coated Vesicle Formation with Single-Molecule Resolution
|
|---|---|
| Chapter number | 15 |
| Book title |
Clathrin-Mediated Endoytosis
|
| Published in |
Methods in molecular biology, August 2018
|
| DOI | 10.1007/978-1-4939-8719-1_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8717-7, 978-1-4939-8719-1
|
| Authors |
François Aguet, Emanuele Cocucci, Aguet, François, Cocucci, Emanuele |
| Abstract |
High-resolution fluorescence microscopy is increasingly contributing to our understanding of molecular processes. By utilizing single-molecule intensity information, imaging experiments can be rendered quantitative, yielding insights into the stoichiometry and kinetics of the components of a molecular assembly. Here, we describe the experimental and analytical steps needed to study the assembly of clathrin-coated vesicles with single-molecule resolution, using total internal reflection fluorescence microscopy. Many components of the protocol are broadly applicable to the characterization of other molecular processes. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 25% |
| Other | 1 | 25% |
| Unknown | 2 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 50% |
| Agricultural and Biological Sciences | 1 | 25% |
| Unknown | 1 | 25% |