You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Purification of Clathrin-Coated Vesicles from Adult Rat Brain
|
---|---|
Chapter number | 1 |
Book title |
Clathrin-Mediated Endoytosis
|
Published in |
Methods in molecular biology, August 2018
|
DOI | 10.1007/978-1-4939-8719-1_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8717-7, 978-1-4939-8719-1
|
Authors |
Silvia Giovedì, Giovedì, Silvia |
Abstract |
Here, we describe a purification protocol for isolating clathrin-coated vesicles (CCVs) from adult rat brain by using differential centrifugation coupled with Ficoll-sucrose and D2O-sucrose density gradient centrifugation and an additional linear sucrose step gradient at the end to separate CCVs from contaminating membranes present in the crude microsomal fraction. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 1 | 25% |
Researcher | 1 | 25% |
Student > Master | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 50% |
Agricultural and Biological Sciences | 1 | 25% |
Unknown | 1 | 25% |