Chapter title |
Imaging “Hot-Wired” Clathrin-Mediated Endocytosis
|
---|---|
Chapter number | 7 |
Book title |
Clathrin-Mediated Endoytosis
|
Published in |
Methods in molecular biology, August 2018
|
DOI | 10.1007/978-1-4939-8719-1_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8717-7, 978-1-4939-8719-1
|
Authors |
Laura A. Wood, Stephen J. Royle, Wood, Laura A., Royle, Stephen J. |
Abstract |
Clathrin-mediated endocytosis (CME) occurs continuously at the plasma membrane of eukaryotic cells. However, when a vesicle forms and what cargo it contains are unpredictable. We recently developed a system to trigger CME on-demand. This means that we can control when endocytosis is triggered and the design means that the cargo that is internalized is predetermined. The method is called hot-wired CME because several steps and proteins are bypassed in our system. In this chapter, we describe in detail how to use the hot-wiring system to trigger endocytosis in human cell lines and how to image the vesicles that form using microscopy and finally, how to analyze those images. |
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Mendeley readers
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