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Clathrin-Mediated Endoytosis

Overview of attention for book
Cover of 'Clathrin-Mediated Endoytosis'

Table of Contents

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    Book Overview
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    Chapter 1 Purification of Clathrin-Coated Vesicles from Adult Rat Brain
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    Chapter 2 Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifugation
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    Chapter 3 Probing Endocytosis During the Cell Cycle with Minimal Experimental Perturbation
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    Chapter 4 Assaying the Contribution of Membrane Tension to Clathrin-Mediated Endocytosis
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    Chapter 5 Identifying Small-Molecule Inhibitors of the Clathrin Terminal Domain
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    Chapter 6 Acute Manipulations of Clathrin-Mediated Endocytosis at Presynaptic Nerve Terminals
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    Chapter 7 Imaging “Hot-Wired” Clathrin-Mediated Endocytosis
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    Chapter 8 Real-Time Endocytosis Measurements by Membrane Capacitance Recording at Central Nerve Terminals
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    Chapter 9 Assaying Mutants of Clathrin-Mediated Endocytosis in the Fly Eye
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    Chapter 10 Reconstitution of Clathrin Coat Disassembly for Fluorescence Microscopy and Single-Molecule Analysis
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    Chapter 11 Spatial and Temporal Aspects of Phosphoinositides in Endocytosis Studied in the Isolated Plasma Membranes
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    Chapter 12 SMrT Assay for Real-Time Visualization and Analysis of Clathrin Assembly Reactions
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    Chapter 13 Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System
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    Chapter 14 Stimulated Emission Depletion (STED) Imaging of Clathrin-Mediated Endocytosis in Living Cells
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    Chapter 15 Measuring Clathrin-Coated Vesicle Formation with Single-Molecule Resolution
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    Chapter 16 Cryo-Electron Tomography of the Mammalian Synapse
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    Chapter 17 Quantitative Analysis of Clathrin-Mediated Endocytosis in Yeast by Live Cell Fluorescence Microscopy
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    Chapter 18 Using FM Dyes to Monitor Clathrin-Mediated Endocytosis in Primary Neuronal Culture
Attention for Chapter 7: Imaging “Hot-Wired” Clathrin-Mediated Endocytosis
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  • Above-average Attention Score compared to outputs of the same age and source (59th percentile)

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Chapter title
Imaging “Hot-Wired” Clathrin-Mediated Endocytosis
Chapter number 7
Book title
Clathrin-Mediated Endoytosis
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8719-1_7
Pubmed ID
Book ISBNs
978-1-4939-8717-7, 978-1-4939-8719-1
Authors

Laura A. Wood, Stephen J. Royle, Wood, Laura A., Royle, Stephen J.

Abstract

Clathrin-mediated endocytosis (CME) occurs continuously at the plasma membrane of eukaryotic cells. However, when a vesicle forms and what cargo it contains are unpredictable. We recently developed a system to trigger CME on-demand. This means that we can control when endocytosis is triggered and the design means that the cargo that is internalized is predetermined. The method is called hot-wired CME because several steps and proteins are bypassed in our system. In this chapter, we describe in detail how to use the hot-wiring system to trigger endocytosis in human cell lines and how to image the vesicles that form using microscopy and finally, how to analyze those images.

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 100%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 September 2018.
All research outputs
#16,094,771
of 24,489,824 outputs
Outputs from Methods in molecular biology
#5,095
of 13,800 outputs
Outputs of similar age
#204,900
of 337,787 outputs
Outputs of similar age from Methods in molecular biology
#93
of 251 outputs
Altmetric has tracked 24,489,824 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,800 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 58% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 337,787 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 251 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.