Chapter title |
Viral Concentration and Amplification from Human Serum Samples Prior to Application of Next-Generation Sequencing Analysis
|
---|---|
Chapter number | 13 |
Book title |
The Human Virome
|
Published in |
Methods in molecular biology, August 2018
|
DOI | 10.1007/978-1-4939-8682-8_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8681-1, 978-1-4939-8682-8
|
Authors |
Xavier Fernandez-Cassi, Marta Rusiñol, Sandra Martínez-Puchol, Fernandez-Cassi, Xavier, Rusiñol, Marta, Martínez-Puchol, Sandra |
Abstract |
The protocol presented here allows the isolation, purification, nucleic acid extraction, and amplification of DNA/RNA from viruses present in human sera samples. The method allows the random amplification of the viral genomes present by using a Sequence-Independent, Single-Primer Amplification (SISPA) approach enabling the study of both DNA/RNA viruses. An amplification step is needed, as the concentration of viral DNA/RNA in serum samples is low for direct library preparation. The application of the described protocol guarantees enough randomly amplified double-strand DNA for further library preparation using Nextera XT kit from Illumina. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 40 | 100% |
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Student > Ph. D. Student | 11 | 28% |
Researcher | 6 | 15% |
Student > Doctoral Student | 3 | 8% |
Student > Master | 3 | 8% |
Professor > Associate Professor | 2 | 5% |
Other | 4 | 10% |
Unknown | 11 | 28% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 4 | 10% |
Environmental Science | 2 | 5% |
Medicine and Dentistry | 2 | 5% |
Agricultural and Biological Sciences | 1 | 3% |
Other | 2 | 5% |
Unknown | 14 | 35% |