| Chapter title |
Protocol for Determination of the Persister Subpopulation in Candida Albicans Biofilms.
|
|---|---|
| Chapter number | 6 |
| Book title |
Bacterial Persistence
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2854-5_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2853-8, 978-1-4939-2854-5
|
| Authors |
De Brucker, Katrijn, De Cremer, Kaat, Cammue, Bruno P A, Thevissen, Karin, Katrijn De Brucker, Kaat De Cremer, Bruno P. A. Cammue, Karin Thevissen, Brucker, Katrijn De, Cremer, Kaat De, Cammue, Bruno P. A. |
| Abstract |
In contrast to planktonic cultures of the human fungal pathogen Candida albicans, C. albicans biofilms can contain a persister subpopulation that is tolerant to high concentrations of currently used antifungals. In this chapter, the method to determine the persister fraction in a C. albicans biofilm treated with an antifungal compound is described. To this end, a mature biofilm is developed and subsequently treated with a concentration series of the antifungal compound of interest. Upon incubation, the fraction of surviving biofilm cells is determined by plating and plotted versus the used concentrations of the antifungal compound. If a persister subpopulation in the biofilm is present, the dose-dependent killing of the biofilm cells results in a biphasic killing pattern. |
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