Chapter title |
Quantitative analysis of the spatiotemporal dynamics of a synthetic predator-prey ecosystem.
|
---|---|
Chapter number | 19 |
Book title |
Synthetic Gene Networks
|
Published in |
Methods in molecular biology, October 2011
|
DOI | 10.1007/978-1-61779-412-4_19 |
Pubmed ID | |
Book ISBNs |
978-1-61779-411-7, 978-1-61779-412-4
|
Authors |
Payne S, Smith RP, You L, Payne, Stephen, Smith, Robert Phillip, You, Lingchong, Stephen Payne, Robert Phillip Smith, Lingchong You |
Abstract |
A major focus in synthetic biology is the rational design and implementation of gene circuits to control dynamics of individual cells and, increasingly, cellular populations. Population-level control is highlighted in recent studies which attempt to design and implement synthetic ecosystems (or engineered microbial consortia). On the one hand, these engineered systems may serve as a critical technological foundation for practical applications. On the other hand, they may serve as well-defined model systems to examine biological questions of broad relevance. Here, using a synthetic predator-prey ecosystem as an example, we illustrate the basic experimental techniques involved in system implementation and characterization. By extension, these techniques are applicable to the analysis of other microbial-based synthetic or natural ecosystems. |
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