| Chapter title |
Reprogramming a GFP Reporter Gene Subjects It to Complex Lentiviral Gene Regulation
|
|---|---|
| Chapter number | 5 |
| Book title |
Synthetic Gene Networks
|
| Published in |
Methods in molecular biology, January 2012
|
| DOI | 10.1007/978-1-61779-412-4_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-411-7, 978-1-61779-412-4
|
| Authors |
Frank Notka, Ralf Wagner |
| Abstract |
Late human immunodeficiency virus (HIV)-derived RNAs encoding relevant therapeutic targets or promising vaccine compounds, such as the HIV-1 group-specific antigen (Gag), are translocated from the nucleus into the cytoplasm via sophisticated export machinery. Relevant steps include the concerted action of several cis-acting RNA elements with the viral Rev-shuttle protein and several cellular components (Ran1/Exportin; Crm1). Based on detailed understanding of the molecular mechanisms guiding this complex process, we used rational codon usage modification to design and reprogram a GFP encoding reporter RNA now exactly mimicking the complex transcriptional and posttranscriptional regulation of late lentiviral mRNAs. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 20% |
| Germany | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 40% |
| Professor | 1 | 20% |
| Researcher | 1 | 20% |
| Student > Doctoral Student | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 40% |
| Agricultural and Biological Sciences | 2 | 40% |
| Medicine and Dentistry | 1 | 20% |