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Chapter title |
Proteomic Analysis of Non-model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis, and MALDI Mass Spectrometry
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Chapter number | 25 |
Book title |
Plant Cell Culture Protocols
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-8594-4_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8593-7, 978-1-4939-8594-4
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Authors |
Petra Peharec Štefanić, Mario Cindrić, Biljana Balen, Peharec Štefanić, Petra, Cindrić, Mario, Balen, Biljana |
Abstract |
Separation of plant proteins by means of electrophoretic techniques is quite challenging since different compounds typical for plant cells can interfere and/or reduce the effectiveness of the protein isolation. This is particularly problematic for two-dimensional electrophoresis (2-DE). Therefore, it is important to optimize protein extraction and to establish a robust protocol for 2-DE and downstream processing, primarily mass spectrometry (MS) analysis. Here we give a detailed protocol for protein extraction using phenol method, 2-DE, and MALDI-MS analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Professor > Associate Professor | 1 | 20% |
Student > Bachelor | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 40% |
Agricultural and Biological Sciences | 2 | 40% |
Unknown | 1 | 20% |